Gene Details:

• Gene ID: glyma.17g053300
• Gene Symbol: GmDGAT1b
• Gene Name: Diacylglycerol acyltransferase
• Genome:
• Species: Glycine max

Functional Descriptions:

• We previously identified a soybean DGAT1b variant modified with 14 amino acid substitutions (GmDGAT1b-MOD) that increases total oil content by 3 percentage points when overexpressed in soybean seeds.
• In the present study, additional GmDGAT1b variants were generated to further increase oil with a reduced number of substitutions. Variants with one to four amino acid substitutions were screened in the model systems Saccharomyces cerevisiae and transient Nicotiana benthamiana leaf.
• One GmDGAT1b variant with three novel amino acid substitutions (GmDGAT1b-3aa) increased total soybean oil to levels near the previously discovered GmDGAT1b-MOD variant. In a multiple location field trial, GmDGAT1b-3aa transgenic events had significantly increased oil and protein by up to 2.3 and 0.6 percentage points, respectively.
• The modeling of the GmDGAT1b-3aa protein structure provided insights into the potential function of the three substitutions. These findings will guide efforts to improve soybean oil content and overall seed composition by CRISPR editing.

Function-related keywords:

• leaf ,  seed ,  oil-content ,  protein

Literature:

• A Novel Soybean Diacylglycerol Acyltransferase 1b Variant with Three Amino Acid Substitutions Increases Seed Oil Content.   DOI:  10.1093/pcp/pcad148 ;  PMID:  37982755

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Orthologs:

Sequences:

• CDS Sequence:
• Protein Sequence: