Gene Details:
Functional Descriptions:
- The β-cyclization of lycopene, catalysed by the lycopene β-cyclases (β-LCY), seems to be a key regulatory step of the carotenoid pathway. In the present study, two β-LCYs from orange fruits (Citrus sinensis), named Csβ-LCY1 and Csβ-LCY2 have been isolated and the activity of the encoded proteins was demonstrated by functional analysis.
- Csβ-LCY1 was expressed at low levels and remained relatively constant during fruit ripening while Csβ-LCY2 showed a chromoplast-specific expression and a marked induction in both peel and pulp of orange fruits in parallel with the accumulation of β,β-xanthophylls.
- Sequence comparison between full-length cDNAs and genomic sequences of Citrus β-LCY1 and β-LCY2 revealed an intronless structure for both genes. DNA blot hybridization with total genomic DNA indicated that both β-LCY1 and β-LCY2 probably exist in a single copy in the C. sinensis genome (data not shown).
- The resulting activities were 95.45±1.20 for Csβ-LCY1 and of 38.33±11.40 for Csβ-LCY2, demonstrating that both genes encoded functional lycopene β-cyclases.
Function-related keywords:
Literature:
- Molecular and functional characterization of a novel chromoplast-specific lycopene beta-cyclase from Citrus and its relation to lycopene accumulation. DOI: 10.1093/jxb/erp048 ; PMID: 19325166
Related News:
Gene Resources:
- NCBI ID: AY094582.1
- UniProt accessions:
Orthologs:
Sequences:
- CDS Sequence:
- Protein Sequence: